Growth of HeLa Cells in Human Adult and Bovine Fetal Serum Medium.

Abstract

HeLa cells cultivated in amino acid-vitamin medium supplemented with human adult and bovine fetal serum, 10% each by volume, grew exponentially during 5 days of incubation in unchanged medium from 48 hours after inoculation. Change in growth rate was associated with change in viability and median cell volume during the growth period. Colonial cultures in mixed and single-serum mediums exhibited differing effects on median cell volume, plating efficiency and cell yield per viable inoculated cell, and on cellular and colonial morphology.