Gel Filtration of Hepatitis B Surface Antigen: Increased size of the native particle
- 1 November 1976
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 33 (2), 345-349
- https://doi.org/10.1099/0022-1317-33-2-345
Abstract
The Stokes radius of unpurified hepatitis B antigen (HBsAg) was determined by chromatography on a carefully calibrated Sepharose 4B column. A value of 14.2 nm was found by this procedure, contrasting with a published value of 11 nm for purified, pepsin-treated HBsAg. Chromatography at pH 3 appeared to reduce the Stokes radius of HBsAg to 11 nm. Serum proteins adsorbed to HBsAg can be removed with pepsin or acid.This publication has 3 references indexed in Scilit:
- Molecular Exclusion and Restricted Diffusion Processes in Molecular-Sieve Chromatography*Biochemistry, 1964
- ANTIGEN DISSOCIATION AS A FACTOR IN IMMUNODIFFUSION ANALYSIS OF HEMOCYANIN1962
- Purification and properties of bacteriophage φX174Journal of Molecular Biology, 1959