Determination of a low pK for histidine-159 in the S-methylthio derivative of papain by proton nuclear magnetic resonance spectroscopy

Abstract

Proton NMR spectroscopy was used to study the ionization behavior of His-159 in a derivative of papain (papain-S-SCH3). In this catalytically inactive derivative of papain, the active-site thiol group of Cys-25 is S-methylthiolated so that it cannot form a thiolate anion. The pH dependence of the chemical shift of the C.epsilon.1 H resonance of His-159 indicated a pK of 3.45 .+-. 0.07 at 45.degree. C in 2H2O with no added ions other than those required for titration. In acetate buffers at an ionic strength of 0.05, the pK increased to 3.87 .+-. 0.12. Conversion of papain-S-SCH3 to active papain at pH* 4.17 (at 45.degree. C and an ionic strength of 0.05) caused the position of the C.epsilon.1 H resonance to change from a position indicative of partial protonation of His-159 to a position indicative of full protonation, consistent with the existence of an imidazolium-thiolate ion-pair interaction between His-159 and Cys-25 in the active enzyme.