IMMUNOLOGICAL DETERMINATION OF PITUITARY LUTEINIZING HORMONE IN THE URINE OF FERTILE AND POST-MENOPAUSAL WOMEN AND ADULT MEN

Abstract

An immunological method to assay human pituitary luteinizing hormone (HPLH) in urine is described. It is based on the fact that HPLH cross-reacts with human chorionic gonadotrophin (HCG) in a hemagglutination inhibition reaction between HCG-coated blood cells and rabbit HCG-antisera. During the menstrual cycle the excretion of HPLH reached a peak of 200-400 U per liter at the time of ovulation. In the urine of post-menopausal women the concentration of HPLH was between 100 and 400 U per liter. In the urine of adult men the concentration of HPLH was between 50 and 160 U per liter.