Observations on the Development ofSetaria labiatopapillosausing New Techniques for InfectingAedes aegyptiwith this Nematode

Abstract

1.The common filarial parasite of cattle in Africa,Setaria labiatopapillosa, has been shown to develop in the mosquitoAedes aegypti. Third stage larvae reached maturity between the 8th and 10th days.2.To avoid feeding mosquitoes on cattle new techniques were developed. Monkeys and rabbits were infected by transplanting adult livingSetariainto then peritoneal cavities. Monkeys, rabbits and rats were also infected by either intraperitoneal or intravenous injections of suspensions of microfilariae extracted from the uteri of adult worms.3.Mosquitoes fed on these substitute hosts were readily infected with theSetaria.4.Mosquitoes were also infected by feeding them on a suspension of microfilariae through membranes of guineapig omentum; the microfilariae were extracted from the uteri of adult worms and suspended in fresh defibrinated rabbit's blood.A few mosquitoes were infected by intrathoracic injections of uterine microfilariae.