Determination of antrycide

Abstract

Antrycide has been determined by reaction with eosin to give a highly fluorescent salt which can be separated from excess eosin by extraction into a mixture of chloroform and butanol. The method measures down to 40 [mu]g. of Antrycide/l. of plasma with satisfactory accuracy, and 20[mu]g./l. approx. The method has a considerable degree of specificity, in that most other tertiary and quaternary bases examined did not react. It can be applied to tissues and urine as well as plasma. A less sensitive method suitable for plasma involves measuring the weak blue fluorescence of Antrycide itself. Two colorimetric methods, suitable only for aqueous solutions, are briefly described.