A SIMPLIFIED PROCEDURE FOR DETERMINATION OF URINARY DEHYDROEPIANDROSTERONE*

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Abstract
When urinary steroids were hydrolyzed by hot-acid procedures, the values for Pettenkofer chromogens in the ketonic fraction did not agree with the values for dehydroepiandrosterone as determined by the Fotherby method. Values for urinary dehydroepiandrosterone determined by measuring the Pettenkofer -reacting material in ketonic extracts of urine after hydrolysis by the two-phase solvolysis procedure of Burstein and Lieberman agreed well with those determined by the chromatographic method of Fotherby. The same results were also obtained in the ketonic extracts of urines prepared by the author''s procedure. The latter method is easier than the chromatographic techniques and shorter than the solvolysis method. Thus it can be recommended as a useful procedure for the routine clinical determination of dehydroepiandrosterone excretion.