On the Turnover of Purines and Pyrimidines from Polynucleotides in the Rat Determined with N15.
- 1 January 1949
- journal article
- research article
- Published by Danish Chemical Society in Acta Chemica Scandinavica
- Vol. 3 (4), 422-432
- https://doi.org/10.3891/acta.chem.scand.03-0422
Abstract
Cytosine and thymine may be prepared from 30-50 mg. desoxyribonucleic acid by hydrolysis with formic acid followed by partition chromatography (I) on starch. Cytidine was converted into uridine by treatment with an aqueous soln. of pyridine, and separation effected by I. While free cytosine may be deaminated to uracil by refluxing with 0 4 [image] H2SO4 for 48 hrs., cytidine was converted mainly into uridine with some uracil and cytosine formed. N15-labelled orotic acid was shown to act as a precursor for both ribo-nucleic acid pyrimidines (II) and desoxyribonucleic acid pyrimidines. On H, the cytosine ring has a higher turnover rate than uracil.This publication has 4 references indexed in Scilit:
- THE DEAMINATION OF CYTIDINE IN ACID SOLUTION AND THE PREPARATION OF URIDINE AND CYTIDINE BY ACID HYDROLYSIS OF YEAST NUCLEIC ACIDJournal of Biological Chemistry, 1949
- THE COMPOSITION OF THE PENTOSE NUCLEIC ACIDS OF YEAST AND PANCREASJournal of Biological Chemistry, 1948
- THE UTILIZATION OF ADENINE FOR NUCLEIC ACID SYNTHESIS AND AS A PRECURSOR OF GUANINEJournal of Biological Chemistry, 1948
- A new form of chromatogram employing two liquid phasesBiochemical Journal, 1941