CELL CYCLE TIME DETERMINATIONS BASED ON LIQUID SCINTILLATION COUNTING OF3H-TdR LABELED MITOTIC CELLS
- 1 July 1977
- journal article
- research article
- Published by Wiley in Cell Proliferation
- Vol. 10 (4), 335-340
- https://doi.org/10.1111/j.1365-2184.1977.tb00301.x
Abstract
Following a 10 min pulse labeling with 3H-TdR, flasks of asynchronous monolayer cultures of Chinese hamster ovary cells were subjected to mitotic selection at 2 h intervals. The mitotic index of the selected populations was always greater than 90%. Counts per min per cell obtained by liquid scintillation counting were plotted vs. time after the pulse label. Comparisons were made between cycle times obtained by the mitotic-scintillation counting method and by the standard per cent labeled mitosis technique. The resulting curves were used for calculations of the cell cycle times and the lengths of G1, S, G2 and M phases of the cell cycle. There was less than 2% difference in the cell cycle times obtained using the scintillation method as compared to times calculated from autoradiographic data obtained from individual petri dishes. The mitotic-scintillation counting technique is simple, accurate and rapid and allows the calculation of the cell kinetics parameters within 1 h of the end of the experiment.This publication has 3 references indexed in Scilit:
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- Timing of replication of the satellite and main band DNAs in cells of the kangaroo rat (Dipodomys ordii)Experimental Cell Research, 1972
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