Regulation of Muscle Contraction: Binding of Troponin and Its Components to Actin and Tropomyosin
Open Access
- 3 March 1975
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 52 (2), 255-263
- https://doi.org/10.1111/j.1432-1033.1975.tb03993.x
Abstract
The binding of troponin components to actin and tropomyosin has been studied by cosedimentation with actin and affinity chromatography. It is shown that troponin binds to actin and tropomyosin in the presence and absence of calcium but the binding to actin is sensitive to ionic strength. Troponin-I + C binds to actin-tropomyosin in the absence of calcium but not to actin or tropomyosin alone. Troponin-I binds to actin and the binding is improved in the presence of tropomyosin even though troponin-I does not bind to tropomyosin alone. Troponin-C does not bind to actin or tropomyosin. The results suggest that the binding of troponin by actin is influenced by tropomyosin. A model of regulation by troponin is proposed.Keywords
This publication has 35 references indexed in Scilit:
- Interactions of troponin subunits with different forms of tropomyosinJournal of Ultrastructure Research, 1974
- Troponin subunit interactionsJournal of Molecular Biology, 1973
- Calcium sensitive binding of troponin to actin-tropomyosin: A two-site model for troponin actionJournal of Molecular Biology, 1973
- Regulation of muscle contraction. Effect of calcium on the affinity of troponin for actin and tropomyosinBiochemistry, 1973
- Structural role of tropomyosin in muscle regulation: Analysis of the X-ray diffraction patterns from relaxed and contracting musclesJournal of Molecular Biology, 1973
- Regulation of skeletal muscle contraction: II. Structural studies of the interaction of the tropomyosin-troponin complex with actinJournal of Molecular Biology, 1972
- Structural changes in actin-containing filaments of muscleJournal of Molecular Biology, 1972
- Activation of the Adenosine Triphosphatase of Limulus polyphemus Actomyosin by TropomyosinThe Journal of general physiology, 1972
- Three-dimensional reconstruction of F-actin, thin filaments and decorated thin filamentsJournal of Molecular Biology, 1970
- Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gelsBiochemical and Biophysical Research Communications, 1967