INVITRO SECONDARY MLR .3. HYBRID HISTOCOMPATIBILITY DETERMINANTS

Abstract

Cells obtained from long-term mixed lymphocyte culture (MLC) were used to demonstrate hybrid histocompatibility determinants that are recognized as mixed lymphocyte reaction (MLR)-stimulating determinants. Hybrid determinants can also be detected in an in vitro assay for effector cells that measure inhibition of immunoglobulin secretion by LPS[lipopolysaccharide]-blasts, and in DTH[delayed hypersensitivity]-like responses of in vitro-primed cells injected into "B" mice and challenged in vivo with stimulator cells. The possible implications of these findings for the physiologic function of MLR determinants are discussed.