Hygromycin B phosphotransferase as a selectable marker for DNA transfer experiments with higher eucaryotic cells.
Open Access
- 1 December 1984
- journal article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 4 (12), 2929-2931
- https://doi.org/10.1128/mcb.4.12.2929
Abstract
The DNA coding sequence for the hygromycin B phosphotransferase gene was placed under the control of the regulatory sequences of a cloned long terminal repeat of Moloney sarcoma virus. This construction allowed direct selection for hygromycin B resistance after transfection of eucaryotic cell lines not naturally resistant to this antibiotic, thus providing another dominant marker for DNA transfer in eucaryotic cells.Keywords
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