The Lipase of Pseudomonas fragi I. Characterization of the Enzyme

Abstract

A modified extraction-titration procedure, using coconut oil as substratum and Na taurocholate as emulsifier, was developed for measurement of lipase activity. Max. lipase activity was at 40[degree]C and pH 7.0-7.2 when a 24-hr. test period was used. Coconut oil was hydrolyzed more rapidly than other natural fats and oils and tricaprylin more rapidly than other simple triglycerides. Complete inactivation of the enzyme required heating at 99[degree] C for 20 min. The enzyme activity could be salted out almost quantitatively by saturation with (NH4)2 SO4, but further purification was not achieved.