A Rapid Procedure for Cloning Genes from λ Libraries by Complementation ofE. coliDefective Mutants: Application to thefabERegion of theE. coliChromosome
- 1 December 1989
- journal article
- research article
- Published by Mary Ann Liebert Inc in DNA
- Vol. 8 (10), 779-789
- https://doi.org/10.1089/dna.1989.8.779
Abstract
I describe a general and rapid procedure allowing the isolation of specialized λ transducing phages from a λ library by lysogenic complementation of defective mutants of Escherichia coli. As an example, the cloning of the E. coli fabE gene and of two other adjacent genetic determinants is presented. Subcloning and determination of its nucleotide sequence reveals that fabE codes for the biotin carboxyl carrier protein (BCCP), one of the three subunits of acetyl coenzyme A carboxylase.This publication has 60 references indexed in Scilit:
- GENETICS AND REGULATION OF BACTERIAL LIPID METABOLISMAnnual Review of Microbiology, 1989
- The E. coli bio operon: Transcriptional repression by an essential protein modification enzymeCell, 1989
- The physical map of the whole E. coli chromosome: Application of a new strategy for rapid analysis and sorting of a large genomic libraryCell, 1987
- Supercoil Sequencing: A Fast and Simple Method for Sequencing Plasmid DNADNA, 1985
- Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectorsGene, 1985
- New versatile cloning and sequencing vectors based on bacteriophage M13Gene, 1983
- Organization of the Escherichia coli chromosome around the genes for translation initiation factor IF2 (infB) and a transcription termination factor (nusA)Journal of Molecular Biology, 1983
- Analysis of bacterial biotin-proteinsBiochimica et Biophysica Acta (BBA) - Protein Structure, 1975
- Calcium-dependent bacteriophage DNA infectionJournal of Molecular Biology, 1970
- Tyrosine-incorporating amber suppressors in Escherichia coli K12Journal of Molecular Biology, 1968