Isolation and structure of a human fibroblast interferon gene
- 1 June 1980
- journal article
- research article
- Published by Springer Nature in Nature
- Vol. 285 (5766), 542-547
- https://doi.org/10.1038/285542a0
Abstract
Chimeric plasmids containing double-stranded c[complementary]DNA copies of mRNA induced in human fibroblasts by poly I .cntdot. C were screened by an RNA selection method. A series of clones to which human fibroblast interferon mRNA selectively hybridized was identified. From the nucleotide sequence of the gene, the complete amino acid sequence of human fibroblast interferon was deduced. The protein is 166 amino acids long and is preceded by a 21-amino acid signal sequence.Keywords
This publication has 50 references indexed in Scilit:
- Coding potential and regulatory signals of the polyoma virus genomeNature, 1980
- Comparison of the Suppression of Cell and Virus Growth in Transformed Human Cells by Leukocyte and Fibroblast InterferonJournal of General Virology, 1979
- Construction and characterization of a plasmid containing a nearly full-size DNA copy of bacteriophage MS2 RNAJournal of Molecular Biology, 1979
- Isolation of separate mRNAs for α- and β-Tubulin and characterization of the corresponding in vitro translation productsCell, 1978
- Translation of mouse interferon mRNA in Xenopus laevis oocytes and in rabbit reticulocyte lysatesBiochemical and Biophysical Research Communications, 1978
- The size of Rous sarcoma virus mRNAs active in cell-free translationNature, 1977
- Is Interferon Tissue Specific? -- Effect of Human Leukocyte and Fibroblast Interferons on the Growth of Lymphoblastoid and Osteosarcoma Cell LinesJournal of General Virology, 1977
- Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase IJournal of Molecular Biology, 1977
- 3′ Non-coding region sequences in eukaryotic messenger RNANature, 1976
- Human Fibroblast and Leukocyte Interferons Show Different Dose-Response Curves in Assay of Cell ProtectionJournal of General Virology, 1976