In order to examine further the relationship between intragastric N-nitrosation, gastric pH and nitrite, 457 fresh, fasting gastric juice samples were analysed for total N-nitroso compounds (NOC) and nitrite concentrations using a recently described improved assay method. Nitrite in log values was linearly related to intragastric pH (r = 0.887, P < 0.01) with a regression equation log [nitrite] (μmol/I) = 0.489 × pH -2.209. Significantly higher NOC concentrations were found at intragastric pH ranges of 1.13–2.99 (mean ± SE: 1.45 ± 0.17 μmol/1, P < 0.05) and 6.00–8.42 (3.57 ± 0.33 μmol/1, P < 0.01) compared with that at pH 3.00–5.99 (1.02 ± 0.12 μmol/I). NOC concentration was significantly related to log nitrite concentration at both the low pH range 1.13–4.99 (r = 0.169, P < 0.01) and the high pH range 5.00–8.42 (r = 0.450, P < 0.01). The results in the present study confirm that both acid-catalysed N-nitrosation and biologically-catalysed N-nitrosation occur in the human stomach. However, great variations in nitrite and NOC concentrations were observed in both low and high pH samples, indicating that, as expected, both the acid-catalysed N-nitrosation and biologically-catalysed N-nitrosation processes are markedly affected by factors other than intragastric pH and nitrite.