Activation of membrane phospholipase C by vasopressin
- 3 February 1986
- journal article
- Published by Wiley in FEBS Letters
- Vol. 196 (1), 155-159
- https://doi.org/10.1016/0014-5793(86)80232-0
Abstract
Vasopressin stimulates the liberation of labelled inositol phosphate in partially purified plasma membranes prepared from myo-[3H]inositol prelabelled WRK1 cells. This stimulatory effect was very rapid (165% stimulation of inositol trisphosphate accumulation after a 10 s incubation period in the presence of 1 μM vasopressin), concentration dependent (EC50= 12 nM) and was abolished by an antagonist of the vasopressor response to vasopressin. GTP, even at high concentrations (0.1 mM), did not increase inositol phosphate release: it was found to be absolutely necessary for hormonal stimulation of phospholipase C activity. Non-hydrolysable analogues of GTP may also stimulate this enzyme activity.Keywords
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