Improved double immunoenzyme labelling using alkaline phosphatase and horseradish peroxidase
Open Access
- 1 October 1982
- journal article
- research article
- Published by BMJ in Journal of Clinical Pathology
- Vol. 35 (10), 1092-1094
- https://doi.org/10.1136/jcp.35.10.1092
Abstract
Alkaline phosphatase and peroxidase complexes were applied to tissue sections simultaneously to label two antigens. An azo-coupling method was used to demonstrate the alkaline phosphatase activity. The final result gave clear contrast between the two antigens and allowed the use of haematoxylin as the nuclear counterstain.This publication has 6 references indexed in Scilit:
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