Isolation, structure and properties of the C‐terminal flanking peptide of preprocholecystokinin from rat brain
- 18 August 1986
- journal article
- Published by Wiley in FEBS Letters
- Vol. 204 (2), 386-390
- https://doi.org/10.1016/0014-5793(86)80849-3
Abstract
The C-terminal flanking peptide of preprocholecystokinin has been isolated from rat brain. Micro-sequence analysis revealed the primary structure: Ser-Ala-Glu-Asp-Tyr-Glu-Tyr-Pro-Ser. Arylsulphatase and mild acid hydrolysis suggested that both tyrosine residues are sulphated. The peptide was not active in bioassay systems that respond to CCK8; the significance of the conserved tripeptide Ser-Ala-Glu is discussed.Keywords
This publication has 12 references indexed in Scilit:
- Cholecystokinin-associated COOH-terminal peptides are fully sulfated in pig brain.Proceedings of the National Academy of Sciences, 1986
- Is the C‐terminal flanking peptide of rat cholecystokinin double sulphated?FEBS Letters, 1986
- Differential expression of the mouse cholecystokinin gene during brain and gut development.Proceedings of the National Academy of Sciences, 1985
- Molecular cloning of the human cholecystokinin gene by use of a synthetic probe containing deoxyinosine.Proceedings of the National Academy of Sciences, 1985
- Molecular Cloning and Nucleotide Sequence of cDNA Coding for Rat Brain Cholecystokinin PrecursorThe Journal of Biochemistry, 1984
- Structural analysis of the gene encoding human gastrin: the large intron contains an Alu sequence.Proceedings of the National Academy of Sciences, 1984
- Cloned cDNA to cholecystokinin mRNA predicts an identical preprocholecystokinin in pig brain and gut.Proceedings of the National Academy of Sciences, 1984
- Structure of a human gastrin gene.Proceedings of the National Academy of Sciences, 1984
- Cloning and sequence analysis of a cDNA encoding rat preprocholecystokinin.Proceedings of the National Academy of Sciences, 1984
- Molecular cloning of the human gastrin geneNucleic Acids Research, 1983