Molecular cloning and chromosomal mapping of a human locus related to the transforming gene of Moloney murine sarcoma virus.
- 1 September 1982
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 79 (17), 5210-5214
- https://doi.org/10.1073/pnas.79.17.5210
Abstract
Human DNA was analyzed for the presence of sequences homologous to the transforming gene (v-mos) of Moloney murine sarcoma virus. A single 2.5-kilobase pair (kbp) EcoRI-generated fragment of human DNA was identified by using cloned v-mos as probe. This DNA was molecularly cloned in a bacteriophage vector. By heteroduplex and restriction enzyme analyses, this human DNA fragment, designated c-mos (human), contained a 0.65-kbp region of continuous homology with v-mos and was present as a single copy in human DNA. By testing for the presence of c-mos (human) in somatic cell hybrids possessing various numbers of human chromosomes, and in subclones of such hybrids, it was possible to assign c-mos (human) to human chromosome 8.This publication has 31 references indexed in Scilit:
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