Age-dependent mitogenesis in normal connective tissue cells
- 1 December 1981
- journal article
- research article
- Published by Springer Nature in Virchows Archiv B Cell Pathology Including Molecular Pathology
- Vol. 36 (1), 27-34
- https://doi.org/10.1007/bf02912051
Abstract
We have previously described ways to use the mesentery for studies of proliferation in intact tissue. Here we have studied a weak and a strong mitogenic response in the mesentery of rats aged 6-42 weeks, induced by a single intraperitoneal injection of saline or the mast-cell-degranulating drug 48/80. Proliferation was measured by the specific DNA activity, the fraction of fibroblast- and mesothelial-like cells in the S + G2 cell-cyclephases, and the mitotic index. We also counted the relative number of mast cells in the tissue (normalized to 5,000 fibroblast-like and mesotheliallike cells), since this might influence 48/80-induced mast-cell-mediated proliferation. In old animals there was a decline in the proliferative response and the time required to initiate DNA synthesis was prolonged. This appears to be the first report of such an age-dependent proliferation characteristic in the mesentery, and probably in any connective tissue. The normalized number of mast cells in the mesentery also declined with increasing age.This publication has 25 references indexed in Scilit:
- Biochemistry of ageingInternational Journal of Biochemistry, 1979
- On serum as a mitogen in intact tissueVirchows Archiv B Cell Pathology Including Molecular Pathology, 1979
- Early changes of cyclic nucleotide levels in a mitogenic reaction in the rat mesenteryCellular and Molecular Life Sciences, 1979
- Protein content, dry mass and chemical composition of individual mast cells related to body growthHistochemistry and Cell Biology, 1979
- Interference of cellular ferric ions with DNA extraction and the application to methods of DNA determinationAnalytical Biochemistry, 1977
- Age-dependent regulation of mammalian DNA synthesis and cell division in vivo by glucocorticoidsExperimental Gerontology, 1974
- Age-dependent regulation of mammalian DNA synthesis and cell proliferation In vivoMechanisms of Ageing and Development, 1972
- An improved fluorometric assay for DNAAnalytical Biochemistry, 1971
- Effects of age on cell proliferation in mouse duodenal cryptsExperimental Gerontology, 1968
- Stimulation of DNA synthesis by isoproterenol in the salivary glandExperimental Cell Research, 1965