Linear and Cyclic α‐Melanotropin [4–10]‐Fragment Analogues That Exhibit Superpotency and Residual Activity

Abstract

Two analogues of .alpha.-MSH (Ac-Ser-Tyr-Ser-Met-Glu-His-Phe-Arg-Trp-Gly-Lys-Pro-Val-NH2), Ac-[Nle4, Asp5, D-Phe7, Lys10].alpha.-MSH4-10NH2 and Ac-[Nle4, Asp5, D-Phe7, Lys10] .alpha.-MSH4-10-NH2, were synthesized, and the melanotropic activities of the peptides were compared in several bioassays. Potencies were determined in the vitro frog and lizard skin bioassays and in the S91 melanoma cell tyrosinase assay. Both analogues were equipotent or more potent than .alpha.-MSH in all bioassays, and the activities of the analogues were prolonged compared to .alpha.-MSH. The two analogues were very resistant to inactivation by purified proteolytic enzymes (.alpha.-chymotrypsin, trypsin, and pepsin). The two peptides could be topically applied and transdermally delivered across the skin of mice in vivo, resulting in a shift from pheomelanogenesis to eumelanogenesis within follicular melanocytes. The cyclic analogue exhibited greater potency, prolonged activity, and stability against enzyme inactivation than did the linear peptide. The significance of the findings for the further design of melanotropin analogues is discussed, as in the possible relevance of these melanotropin analogues for use in biomedical studies.