Stimulation of RNA Polymerase II Elongation by Hepatitis Delta Antigen
- 6 July 2001
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 293 (5527), 124-127
- https://doi.org/10.1126/science.1057925
Abstract
Transcription elongation by RNA polymerase II (RNAPII) is negatively regulated by the human factors DRB-sensitivity inducing factor (DSIF) and negative elongation factor (NELF). A 66-kilodalton subunit of NELF (NELF-A) shows limited sequence similarity to hepatitis delta antigen (HDAg), the viral protein required for replication of hepatitis delta virus (HDV). The host RNAPII has been implicated in HDV replication, but the detailed mechanism and the role of HDAg in this process are not understood. We show that HDAg binds RNAPII directly and stimulates transcription by displacing NELF and promoting RNAPII elongation. These results suggest that HDAg may regulate RNAPII elongation during both cellular messenger RNA synthesis and HDV RNA replication.Keywords
This publication has 23 references indexed in Scilit:
- Positive Transcription Elongation Factor b Phosphorylates hSPT5 and RNA Polymerase II Carboxyl-terminal Domain Independently of Cyclin-dependent Kinase-activating KinaseJournal of Biological Chemistry, 2001
- HIV-1 Tat: coping with negative elongation factorsCurrent Opinion in Immunology, 1999
- Comparative Analysis of a Novel Gene from the Wolf–Hirschhorn/Pitt–Rogers–Danks Syndrome Critical RegionGenomics, 1999
- Inhibition of Cellular RNA Polymerase II Transcription by Delta Antigen of Hepatitis Delta VirusVirology, 1998
- A Cellular Homolog of Hepatitis Delta Antigen: Implications for Viral Replication and EvolutionScience, 1996
- How Did Replicating and Coding RNAs First Get Together?Science, 1996
- THE MOLECULAR BIOLOGY OF HEPATITIS DELTA VIRUSAnnual Review of Biochemistry, 1995
- Prediction of Protein Secondary Structure at Better than 70% AccuracyJournal of Molecular Biology, 1993
- The large form of hepatitis delta antigen is crucial for assembly of hepatitis delta virus.Proceedings of the National Academy of Sciences, 1991
- The classification of amino acid conservationJournal of Theoretical Biology, 1986