Tuberculin Reactivity of a Carbohydrate Component of Unheated BCG Culture Filtrate

Abstract
Carbohydrate and protein fractions from the filtrate of a culture of Mycobacterium bovis strain BCG have equal activity in sensitive guinea pigs. The sequential action of two proteolytic enzymes caused little alteration in the reactivity of the carbohydrate but almost completely eliminated the reactivity of the protein.