The estimation of serum bilirubin

Abstract

A method is descr. for determining bilirubin in serum which does not involve separation of protein. Color is developed by the van den Bergh reagent in the presence of a urea-ethanol soln., and clarity achieved by use of phenol, following the inactivation of excess diazonium compound. The results achieved by the present method are in closer alignment with those of Malloy and Evelyn (1937) than with those of King and Coxon (1950). As compared with the method of Malloy and Evelyn, the present technic is capable of giving greater accuracy at low bilirubin levels.