Isocitratase, which cleaves isocitrate to glyoxylate and succinate and is a key enzyme in the glyoxylate cycle, is formed by Rhodopseudomonas palustris and R. capsulatus when grown on acetate or butyrate either anaerobically in light or aerobically in the dark. Only traces of the enzyme are present in organisms grown on succinate or malate. In contrast, isocitratase is detectable in traces only in R. spheroides and Rhodospirillum rubrum grown on acetate, butyrate or other substrates. This suggests that the glyoxylate cycle cannot account for net synthesis of cell constituents from acetate or acetate precursors in these latter two organisms.