MOLECULAR POPULATION GENETICS OF THE ALCOHOL DEHYDROGENASE GENE REGION OF DROSOPHILA MELANOGASTER
Open Access
- 30 November 1986
- journal article
- research article
- Published by Oxford University Press (OUP) in Genetics
- Vol. 114 (4), 1165-1190
- https://doi.org/10.1093/genetics/114.4.1165
Abstract
Variation in the DNA restriction map of a 13-kb region of chromosome ll including the alcohol dehydrogenase structural gene (Adh) was examined in Drosophila melanogaster from natural populations. Detailed analysis of 48 D. melanogaster lines representing four eastern United States populations revealed extensive DNA sequence variation due to base substitutions, insertions and deletions. Cloning of this region from several lines allowed characterization of length variation as due to unique sequence insertions or deletions [nine sizes; 21-200 base pairs (bp)] or transposable element insertions (several sizes, 340 bp to 10.2 kb, representing four different elements). Despite this extensive variation in sequences flanking the Adh gene, only one length polymorphism is clearly associated with altered Adh expression (a copia element approximately 250 bp 5′ to the distal transcript start site). Nonetheless, the frequency spectra of transposable elements within and between Drosophila species suggests they are slightly deleterious. Strong nonrandom associations are observed among Adh region sequence variants, ADH allozyme (Fast vs. Slow), ADH enzyme activity and the chromosome inversion ln(2L)t. Phylogenetic analysis of restriction map haplotypes suggest that the major twofold component of ADH activity variation (high vs. low, typical of Fast and Slow allozymes, respectively) is due to sequence variation tightly linked to and possibly distinct from that underlying the allozyme difference. The patterns of nucleotide and haplotype variation for Fast and Slow allozyme lines are consistent with the recent increase in frequency and spread of the Fast haplotype associated with high ADH activity. These data emphasize the important role of evolutionary history and strong nonrandom associations among tightly linked sequence variation as determinants of the patterns of variation observed in natural populations.This publication has 67 references indexed in Scilit:
- Lambda replacement vectors carrying polylinker sequencesJournal of Molecular Biology, 1983
- A family of oligo-adenylate-terminated transposable sequences in Drosophila melanogasterJournal of Molecular Biology, 1983
- Evolution of single-copy DNA and the ADH gene in seven drosophilidsJournal of Molecular Evolution, 1982
- FB elements are the common basis for the instability of the wDZL and wc Drosophila mutationsCell, 1982
- Genetic variation at the alcohol dehydrogenase locus inDrosophila melanogaster: A third ubiquitous alleleCellular and Molecular Life Sciences, 1982
- Evolution of the β-globin gene cluster in man and the primatesJournal of Molecular Biology, 1981
- Eucaryotic transposable genetic elements with inverted terminal repeatsCell, 1980
- Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase IJournal of Molecular Biology, 1977
- Detection of specific sequences among DNA fragments separated by gel electrophoresisJournal of Molecular Biology, 1975
- fferences in the number of molecules produced by two allelic electrophoretic enzyme variantsD. melanogasterCellular and Molecular Life Sciences, 1972