The Technique of Quantitative Chorioallantoic Virus Titration

Abstract

The technique of quantitative virus titration of the chorioallantois has been modified to avoid as far as possible the nonspecific lesions produced by hemorrhage when the chorioallantois is separated from the shell membrane prior to inoculation. A drop of sterile saline is used as a fluid wedge to facilitate this separation. A number of other technical points in the processes of inoculating, removing and counting the membranes are discussed.