COMPARISON OF PAPER AND THIN-LAYER RADIOCHROMATOGRAPHY FOR THE QUANTITATIVE ANALYSIS OF THYROIDAL IODOAMINO ACIDS

Abstract

SUMMARY: Paper and thin-layer radiochromatography have been applied both to the analysis of iodine-containing compounds discharged by mouse thyroid glands maintained in vitro and to their distribution in hydrolysates of the gland. The results are in close agreement. The addition of thyroid-stimulating hormone to the medium supporting the mouse thyroid glands increased the release of labelled iodothyronines. Compared with paper, thin-layer chromatography separated thyroxine and tri-iodothyronine more effectively; there was also less deiodination and a higher degree of reproducibility and reliability.