Abstract
Ovary development of worker bees increased progressively in colonies receiving the following treatments: (a) with a queen and her brood, or without a queen but with laying worker bees and queen brood, or without a queen but with laying worker bees and their (drone) brood (in one of two experiments); (b) with a queen but without brood; (c) with laying worker bees but without brood; (d) without a queen, laying worker bees, or brood of any kind.