In Vitro Synthesis of Turnip Yellow Mosaic Virus Coat Protein in a Wheat Germ Cell-Free System
- 1 October 1976
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 20 (1), 196-202
- https://doi.org/10.1128/jvi.20.1.196-202.1976
Abstract
Turnip Yellow Mosaic Virus RNA directs the synthesis in vitro of its coat protein in a wheat germ cell-free extract. Optimum conditions for synthesis were defined and the effect of spermine on specifically enhancing coat protein formation was examined. Identity between the in vitro synthesized coat protein and authentic coat protein of Turnip Yellow Mosaic Virus was established by analysis on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, peptide mapping and immunoprecipitation.This publication has 28 references indexed in Scilit:
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