Antilipolytic Effects of Sulfonylurea Drugs on Induced Lipolysis in Isolated Fat Cells of the Rat1

Abstract

The effect of tolbutamide and tolazamide on fatty acid and glycerol release in vitro was examined in fat cells isolated from epididymal adipose tissue of fasted rats. Incubations were performed in the absence of glucose. Tolbutamide reduced basal rate of release of both glycerol and fatty acid. Both tolbutamide and tolazamide blocked the lipolytic effect of DL-arterenol, ACTH, theophylline and glucagon. Tolbutamide inhibited the lipolytic effect of dexamethasone and growth hormone whether it was added initially or 2 hr. after the start of incubation. Anti-insulin serum, demonstrated to inhibit the antilipolytic effect of insulin, did not in-hibit the antilipolytic effect of tolbutamide.