SPONTANEOUS RELEASE OF T-CELL RECEPTORS FOR ALLOANTIGENS
Open Access
- 1 September 1974
- journal article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 140 (3), 603-618
- https://doi.org/10.1084/jem.140.3.603
Abstract
In vitro cultivation of murine spleen cells resulted in a spontaneous release of receptors for alloantigens. This was revealed by the capacity of cell-free culture supernates to recognize alloantigens as measured in the PAR assay. Qualitatively, recognition responses obtained with these supernates reproduced faithfully those found with the corresponding cells. Large amounts of receptors were released by untreated spleen cells and by spleen cells treated with a rabbit antimouse immunoglobulin serum and complement, smaller amounts were released by bone marrow cells, and native thymus cells released none. Spleen cells from nude mice and spleen cells from normal mice treated with anti-theta serum and complement showed no release of receptors. It was concluded that receptors active in the PAR test were of T-cell origin. Release of T-cell receptors was found to be discontinuous and proceeded in waves. The amount of released receptors depended on the number of cells cultivated. Release occurred at 37 degrees C but not at 4 degrees C. Interaction with antigen, however, was temperature-independent. In contrast to T-cell receptors, a release of H-2 antigens could not be detected with the culture conditions employed.Keywords
This publication has 22 references indexed in Scilit:
- ISOLATION AND PARTIAL CHARACTERIZATION OF LYMPHOCYTE SURFACE IMMUNOGLOBULINSThe Journal of Experimental Medicine, 1972
- QUANTITATIVE FEATURES OF A SANDWICH RADIOIMMUNOLABELING TECHNIQUE FOR LYMPHOCYTE SURFACE RECEPTORSThe Journal of Experimental Medicine, 1972
- Interaction between lymphocytes in immune responsesCellular Immunology, 1971
- IMMUNOGLOBULIN AND OTHER SURFACE ANTIGENS OF CELLS OF THE IMMUNE SYSTEMThe Journal of Experimental Medicine, 1971
- IMMUNOGLOBULINS ON THE SURFACE OF LYMPHOCYTESThe Journal of Experimental Medicine, 1971
- Immunological responses of 'nude' mice.1971
- Immunological unresponsiveness of genetically thymusless (nude) miceEuropean Journal of Immunology, 1971
- Quantitative studies on antigenic recognition. II. Kinetics of the recognition eventEuropean Journal of Immunology, 1971
- Specific in vitro Cytotoxicity of Thymus-derived Lymphocytes sensitized to AlloantigensNature, 1970
- QUANTITATIVE STUDIES ON ANTIGENIC RECOGNITIONThe Journal of Experimental Medicine, 1969