RECONSTITUTION OF AMYLOID FIBRILS FROM ALKALINE EXTRACTS

Abstract

Amyloid fibrils were extracted with NH4OH and other solvents at high alkaline pH (pH 11.5), and then precipitated by reducing the pH to 4-5 with 1-2 N HCl. The precipitate showed tinctorial and ultrastructural properties identical with those of native amyloid. Attempts were also made to obtain this derivative in purer form by using a fractional extraction scheme. These methods are useful in the purification and reconstitution of the amyloid fibril and its sub-units.