Effects of Multiplicity of Poliovirus Infection of HeLa Cells Upon Regulation of DNA Synthesis.

Abstract

The rate of DNA synthesis in HeLa cells cultivated in medium without histidlne was stimulated in the early stages of infection with pollovlrus and could be inhibited in the late stages. The degree of each effect increased with increasing multiplicities of infection. The inhibition, but not the stimulation, was prevented with guanidlne. The effect of guanldlne was blocked by dlmethylaminoethanol. Both viral effects upon DNA synthesis which are dependent upon the multiplicity of infection are indicators of 1 primary interaction of the parental vlrion with some cell structure.