Analysis of Rye Pollen (Secale cereale) Allergens Using Patients’ IgE, Immunoprint, Western Blot and Monoclonal Antibodies

Abstract

Rye pollen (Secale cereale) was investigated by Immunoprint and Western Blot analysis. Using Immunoprint, more than 30 proteins could be shown to be allergens in terms of their IgE binding, and 9 were determined as major allergens. In Western Blot 17 allergens were discovered, 3 of them major allergens. 4 monoclonal antibodies raised against a pollen extract of 6 grasses showed cross-reactivity to rye pollen, thus indicating common epitopes. 1 of 3 monoclonal antibodies produced against an extract of rye pollen was only reacting to this pollen. Looking at the overall binding pattern of the 7 monoclonal antibodies, they showed partial identity to patients’ IgE in Immunoprint and Western Blot. The possibility of defining epitopes using preparative isolelectric focusing and monoclonal antibodies is discussed.