The Energy‐State of Mitochondria during the Transport of Ca2+
- 1 September 1980
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 110 (1), 211-216
- https://doi.org/10.1111/j.1432-1033.1980.tb04857.x
Abstract
The fluctuations of the membrane potential during mitochondrial Ca2+ transport have been monitored with an electrode sensitive to tetraphenylphosphonium. The following conclusions have been reached. 1. The membrane becomes depolarized during the influx of Ca2+. When the bulk of the Ca2+ pulse has been taken up, it repolarizes, but not completely. 2. If all of the accumulated Ca2+ is released from mitochondria and cycling is inhibited, the membrane repolarizes completely. 3. The accumulation of Ca2+ alone does not induce mitochondrial damage. In the presence of inorganic phosphate, the uptake of Ca2+ may lead to complete and irreversible depolarization, depending on the amount of Ca2+ and phosphate accumulated. The irreversible damage observed in the presence of phosphate is prevented by Mg2+.Keywords
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