Spectroscopic studies of oxy- and carbonmonoxyhemoglobin after pulsed optical excitation.

Abstract
The photolysis of [human] HbO2 and HbCO was investigated with ps laser techniques. Transient absorption spectra were measured in the Soret and visible regions after excitation with 353- or 530-nm pulses. The photoproducts appeared within 8 ps and exhibited considerably broadened deoxyhemoglobin-like spectra, which persisted to 680 ps. The altered spectra were attributed to the production of deoxyheme conformational and spin states that might result from intense excitation.