THIN-LAYER CHROMATOGRAPHIC DETERMINATION OF AFLATOXIN-B1 IN EGGS - COLLABORATIVE STUDY

Abstract

The TLC method of Trucksess et al. for aflatoxin B1 in [chicken] eggs was collaboratively studied. Each collaborator analyzed 3 known practice samples and 9 unknown samples containing added aflatoxin B1 at 0, 0.05, 0.10 and 0.30 ng/g. For 9 collaborators, recoveries for the 3 positive levels were: 0-0.13 ng/g (average 98%, coefficient of variation (C.V.) 83%), 0.05-0.18 ng/g (average 102%, C.V. 36%) and 0.11-0.42 ng/g (average 93%, C.V. 31%), respectively. The method was adopted as official first action.