Acetylcholine Responses in L Cells
- 15 September 1972
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 177 (4053), 1005-1007
- https://doi.org/10.1126/science.177.4053.1005
Abstract
L cells, a family of continuous cell lines of mouse fibroblastic origin, generate a prolonged active membrane hyperpolarization (the hyperpolarizing activation response) when stimulated mechanically or electrically. lontophoretically applied acetylcholine elicits a similar response; atropine blocks the acetylcholine but not the electrically or mechanically elicited responses. The hyperpolarizing activation response can also be elicited by electrical, mechanical, or acetylcholine stimulation of cells adjacent to the recorded cell. Propagation of the response from one cell to another is not dependent on direct electrical coupling between cells and is not blocked by application of a bath containing atropine or curare. These results show that L cells are capable of generating an active electrical response. that they are sensitive to at least one neurotransmitter (acetylcholine), and that humorally mediated interaction (probably noncholinergic) between L cells occurs.Keywords
This publication has 8 references indexed in Scilit:
- An Active Electrical Response in FibroblastsThe Journal of general physiology, 1972
- Responses of neuroblastoma cells to iontophoretically applied acetylcholineJournal of Cellular Physiology, 1971
- Electrogenesis in mouse neuroblastoma cells In vitroJournal of Cellular Physiology, 1971
- Conversion of synaptic excitation to inhibition at a dual chemical synapse.Journal of Neurophysiology, 1971
- Acetylcholine Sensitivity and Distribution on Mouse Neuroblastoma CellsScience, 1970
- Generation of Adrenergic and Cholinergic Potentials in Sympathetic Ganglion CellsScience, 1969
- Effects of acetylcholine and other medullary secretagogues and antagonists on the membrane potential of adrenal chromaffin cells: an analysis employing techniques of tissue cultureThe Journal of Physiology, 1967
- Electrophysiology of Salivary GlandsPhysiological Reviews, 1958