Separation of Bacterial Menaquinones by HPLC Using Reverse Phase (RP18) and a Silver Loaded Ion Exchanger as Stationary Phases

Abstract

Bacterial menaquinones were separated isocratically on a reverse phase Li Chrosorb RP18 5 μm and a silver loaded ion exchanger. On octyldecylsilica support the separation of the menaquinones depends on their lipophilic character, on the silver column mainly on the number of double bonds in the isoprenyl chain. Comparing the runs of both columns the menaquinones were easily differentiated.