Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Direct Bacterial Identification from Positive Blood Culture Pellets
Top Cited Papers
- 1 April 2010
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 48 (4), 1481-1483
- https://doi.org/10.1128/jcm.01780-09
Abstract
An ammonium chloride erythrocyte-lysing procedure was used to prepare a bacterial pellet from positive blood cultures for direct matrix-assisted laser desorption-ionization time of flight (MALDI-TOF) mass spectrometry analysis. Identification was obtained for 78.7% of the pellets tested. Moreover, 99% of the MALDI-TOF identifications were congruent at the species level when considering valid scores. This fast and accurate method is promising.Keywords
This publication has 7 references indexed in Scilit:
- Ongoing Revolution in Bacteriology: Routine Identification of Bacteria by Matrix‐Assisted Laser Desorption Ionization Time‐of‐Flight Mass SpectrometryClinical Infectious Diseases, 2009
- Evaluation of Matrix-Assisted Laser Desorption Ionization-Time-of-Flight Mass Spectrometry in Comparison to 16S rRNA Gene Sequencing for Species Identification of Nonfermenting BacteriaJournal of Clinical Microbiology, 2008
- Highly Efficient Classification and Identification of Human Pathogenic Bacteria by MALDI-TOF MSMolecular & Cellular Proteomics, 2008
- Rapid Identification of Viridans Streptococci by Mass Spectrometric DiscriminationJournal of Clinical Microbiology, 2007
- Detection and Treatment of Bloodstream Infection: Laboratory Reporting and Antimicrobial ManagementJournal of Clinical Microbiology, 2003
- Sample preparation of Gram-positive bacteria for identification by matrix assisted laser desorption/ionization time-of-flightJournal of Microbiological Methods, 2002
- Phyloproteomics: species identification of Enterobacteriaceae using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.2001