The repair patch ofE.coli(A)BC excinuclease
Open Access
- 11 September 1990
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 18 (17), 5051-5053
- https://doi.org/10.1093/nar/18.17.5051
Abstract
The size of repair patch made by E. coli DNA polymerase I (Poll) following the removal of a thymine-psoralen monoadduct by E. coli (A)BC excinuclease was determined by using an M13mp19 DNA with a single psoralen monoadduct at the polylinker region. Incubation of this substrate with (A)BC excinuclease, Poll and a combination of 3 dNTP plus 1 dNTP(αS) for each nucleotide, and DNA ligase resulted in a repair patch with phosphorothioate linkages. The preferential hydrolysis of phosphorothioate bonds by heating in iodoethanol revealed a patch size-with minimal nick translation-equal in length to the 12 nucleotide gap generated by this excision nuclease.Keywords
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