Improved techniques for the induction of mammalian cell hybridization by polyethylene glycol
- 1 March 1976
- journal article
- research article
- Published by Springer Nature in Somatic Cell and Molecular Genetics
- Vol. 2 (2), 165-176
- https://doi.org/10.1007/bf01542629
Abstract
Modifications in the techniques for the induction of mammalian somatic cell hybridization by polyethylene glycol (PEG) have led to procedures that are rapid, simple, and effective. The basic improvements, for both monolayer and suspension fusions, are a short exposure to PEG and a rapid dilution of PEG following treatment. There is a marked effect of PEG concentration on cell hybridization, and there seem to be inherent differences between cells in terms of the extent of cell fusion induced by PEG.Keywords
This publication has 15 references indexed in Scilit:
- Production of mammalian somatic cell hybrids by means of polyethylene glycol treatmentSomatic Cell and Molecular Genetics, 1975
- Isolation of ouabain-resistant human diploid fibroblastsCell, 1974
- Agglutination and fusion of plant protoplasts by polyethylene glycolCanadian Journal of Botany, 1974
- Plant protoplast fusion and growth of intergeneric hybrid cellsPlanta, 1974
- A method for high-frequency intergeneric fusion of plant protoplastsPlanta, 1974
- Regulation of specific functions of glial cells in somatic hybrids. I. Control of S100 proteinJournal of Cellular Physiology, 1971
- Induction of homokaryocyte, heterokaryocyte and hybrid formation by lysolecithinExperimental Cell Research, 1971
- Factors influencing the “effective mating rate” of mammalian cellsExperimental Cell Research, 1970
- Regulation of melanin synthesis in mammalian cells, as studied by somatic hybridization: III. A method of increasing the frequency of cell fusionExperimental Cell Research, 1969
- Selection of Hybrids from Matings of Fibroblasts in vitro and Their Presumed RecombinantsScience, 1964