Monitoring and identification of residues of anabolic preparations in slaughtered cattle by HPLC with diode array detection
Open Access
- 1 April 1984
- journal article
- research article
- Published by Taylor & Francis in Veterinary Quarterly
- Vol. 6 (2), 60-65
- https://doi.org/10.1080/01652176.1984.9693913
Abstract
The new combination of isocratic high performance liquid chromatography (HPLC) with on line UV spectrum detection via a diode array configuration has been applied to the detection and identification of anabolics present in application sites of cattle. Combination of the characteristic retention time in the HPLC chromatogram and a comparison of the full spectrum between 190–400 nm of the anabolic components with that of a standard resulted in a very reliable identification. By means of this method 117 samples of application sites were investigated for the presence of anabolic residues. Of the xenobiotic anabolics, 19‐nortestosterone (NT) was found most frequently (in 96 cases), whereas diethylstilbestrol (DES) was found in only 11 cases. In all samples the identification of NT and DES was confirmed by high resolution gas chromatography‐mass spectrometry (GCMS).Keywords
This publication has 4 references indexed in Scilit:
- Identification of several stilbene derivatives in bovine urine by means of high performance liquid chromatographic fractionation and immunochemical detectionVeterinary Quarterly, 1984
- Identification and quantification of diethylstilbestrol in the urine of cattle by both low and high resolution gas chromatography mass spectrometryJournal of Mass Spectrometry, 1983
- Modellversuch zur Beurteilung der Rückstandssituation nach intramuskulärer Verabreichung einer kristallinen Suspension von Diäthylstilböstroldipropionat an MastkälberZentralblatt für Veterinärmedizin Reihe A, 1982
- Weight increase of the thyroid gland as a tentative screening parameter to detect the illegal use of thyreostatic compounds in slaughter cattleVeterinary Quarterly, 1982