Sulphoxidation of albendazole by the FAD-containing and cytochrome P-450 dependent mono-oxygenases from pig liver microsomes
- 1 January 1987
- journal article
- research article
- Published by Taylor & Francis in Xenobiotica
- Vol. 17 (10), 1159-1168
- https://doi.org/10.3109/00498258709167408
Abstract
1. Two distinct microsomal pathways involved in the metabolism of albendazole (ABZ) to albendazole-sulphoxide (SO.ABZ) by pig liver microsomes have been identified and quantified. 2. The binding of ABZ to microsomal cytochrome P-450 (Type I spectrum, Ks = 25.5 microM), the decrease of the rate of sulphoxidation by antibody against NADPH cytochrome c reductase, and the use of purified cytochrome P-450 A demonstrated the contribution of a cytochrome P-450-dependent mono-oxygenase to the metabolism of ABZ. 3. The involvement of FAD-containing mono-oxygenase (FMO) was shown by thermal pretreatment of microsomes, n-octylamine activation of the reaction, and by using purified pig liver FMO. 4. From Km and Vmax values, it would appear that the relative contributions of the two systems depend on the concentration of ABZ.Keywords
This publication has 21 references indexed in Scilit:
- Comparison of sulfide oxygenation mechanism for liver microsomal FAD-containing monooxygenase with that for cytochrome P-450Biochemical and Biophysical Research Communications, 1985
- The Early History of Xenobiotic SulfoxidationDrug Metabolism Reviews, 1985
- A correlation of toxicity of albendazole and oxfendazole with their free metabolites and bound residuesJournal of Veterinary Pharmacology and Therapeutics, 1984
- Limitations on the metyrapone assay for the major phenobarbital inducible form of cytochrome P-450Biochemical and Biophysical Research Communications, 1982
- Immunochemical characterization of some monooxygenase activities in liver microsomes from untreated and phenobarbital-treated ratsBiochemical and Biophysical Research Communications, 1981
- Some Properties of the Sulphoxidases and Sulphoxide Reductases of the CestodeMoniezia Expansa, the NematodeAscaris Suumand Mouse LiverXenobiotica, 1979
- Anthelmintic activity of albendazole against liver flukes, tapeworms, lung and gastrointestinal roundwormsCellular and Molecular Life Sciences, 1976
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- Nature of the type I and II spectral changes in liver microsomesBiochemistry, 1970
- Ligand interactions with cytochrome P-450. I. Binding of primary aminesBiochemistry, 1969