Long-Term High-Titer Neutralizing Activity Induced by Octameric Synthetic HIV-1 Antigen
- 11 October 1991
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 254 (5029), 285-288
- https://doi.org/10.1126/science.1925584
Abstract
A titer for homologous viral neutralization activity (greater than 1:19,683) was observed after a 3.5-year immunization period with an octameric, branching peptide representing the principal neutralizing determinant (PND) of the human immunodeficiency virus-1IIIB envelope protein. Booster immunizations elicited persistent and potent antibodies in guinea pigs, exceeding responses produced by a conventional bovine serum albumin conjugate by 100-fold. Peptide length, central presentation of a conserved sequence, and inclusion of an upstream sequence contributed to immunogenicity. Titers (greater than 1:1,000) of heterotypic neutralizing antibodies also developed. Octameric PND peptides are a promising approach for an acquired immunodeficiency syndrome (AIDS) vaccine.Keywords
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