Estrogen Receptors in Cultured Rat Uterine Cells: Induction of Progesterone Receptors in the Absence of Estrogen Receptor Processing*
- 1 February 1986
- journal article
- research article
- Published by The Endocrine Society in Endocrinology
- Vol. 118 (2), 603-608
- https://doi.org/10.1210/endo-118-2-603
Abstract
When cultured rat uterine cells were treated for up to 6 h with 5 nM 17.beta.-estradiol, no decrease in the [3H] estradiol-binding capacity of the cells was observed (i.e. no processing). This was true whether the cells were treated directly with 5 nM [3H]estradiol or with 5 nM unlabeled 17.beta.-estradiol followed by homogenization and exchange with [3H]estradiol in vitro. In additional experiments, intact cells were treated with medium containing 5 nM [3H]estradiol for 30 min, and then that medium was removed and replaced with medium containing 5 nM unlabeled 17.beta.-estradiol. Receptor-bound estradiol in intact cells was totally exchangeable with estradiol in the culture medium (t1/2, .apprx. 90 min). Six-hour treatment of cells with 5 nM 17.beta.-estradiol led to a 50% increase in the [3H]progesterone-binding capacity of the cells, while no loss of estrogen-binding capacity occurred. These results indicate that progesterone receptors can be induced by estrogen in the rat uterus in the absence of estrogen receptor processing.This publication has 17 references indexed in Scilit:
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