Umbelliferone Phosphate as a Substrate for Acid and Alkaline Phosphatase

Publisher Website
Google Scholar
Abstract
The results of a complete study of 8 substrates for acid and alkaline phosphatase indicated 7-hydroxycoumarin (umbelliferone) phosphate to be the best substrate for the analysis of these enzymes. Using this ester, from 10−6 to 2 × 10−2 units per ml. of alkaline phosphatase and 10−5 to 0.06 units per ml. of acid phosphatase can be determined with an accuracy and precision of about 1.5%. Samples of serum as small as 1 μ;1. can be assayed. Analysis is performed by a direct initial reaction rate method in 2–3 minutes.