O-aryl S-methyl phosphorochloridothioates: terminal phosphorylating agents in the phosphotriester approach to oligonucleotide synthesis

Abstract

O-Aryl S-methyl phosphorothioates (e.g., 6), prepared by the action of O-aryl S-methyl phosphorochloridothioates (e.g., 4c) on alcohols may be converted into the corresponding phosphate esters (e.g., 7b) by treatment first with 4-nitrobenzaldoximate ion and then with iodine in aqueous pyridine.